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ATCC accession number crl 1569
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ATCC cell lines ramos atcc crl 1569 u937 atcc crl 1593 2 c4 2 md anderson n a u 2 os atcc htb 96 hela atcc ccl
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ATCC cell lines human breast cancer cell lines
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Journal: Cell systems

Article Title: ROS and Oxidative Stress are Elevated in Mitosis During Asynchronous Cell Cycle Progression and are Exacerbated by Mitotic Arrest

doi: 10.1016/j.cels.2019.01.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho S10 histone H3 [3H10] EMD Millipore Cat#05-806 Cysteine sulfenic acid (dimedone) EMD Millipore Cat#07-2139 8-oxoguanine clone 483.15 EMD Millipore Cat#MAB3560 β-actin (rabbit) Cell Signaling Technologies Cat#4967 Bacterial and Virus Strains None Biological Samples None Chemicals, Peptides, and Recombinant Proteins DMEM Corning CellGro TM Cat#10-017-CV RPMI-1640 Gibco TM Cat#11875-093 Fetal Bovine Serum (FBS) Seradigm Cat#1500-500 chloromethyl 2′,7′-dichlorodihydrofluorescein diacetate (CM-H 2 DCFDA) Invitrogen Cat#C6827 BI2536 Selleck Chemicals Cat#S1109 Alisertib Selleck Chemicals Cat#S1133 Nocodazole Sigma Aldrich Cat#M1404 5,5-dimethyl-1,3-cyclohexanedione (dimedone) Sigma Aldrich Cat#38490 iodoacetamide Sigma Aldrich Cat#I-1149 Docetaxel LC Laboratories Cat#D-1000 4,6-diamidino-2-phenylindole (DAPI) Molecular Probes Cat#D1306 Critical Commercial Assays None Deposited Data None Experimental Models: Cell Lines Ramos ATCC CRL-1569 U937 ATCC CRL-1593.2 C4-2 MD Anderson N/A U-2 OS ATCC HTB-96 HeLa ATCC CCL-2 Experimental Models: Organisms/Strains None Oligonucleotides None Recombinant DNA None Software and Algorithms FlowJo version 7.6.5 FlowJo, LLC Seaborn statistical data visualization version 0.7.1 http://seaborn.pydata.org Other None Open in a separate window KEY RESOURCES TABLE ROS varies with the cell cycle in freely-cycling cancer cells ROS levels peak in G2 and mitosis Oxidation of biomolecules is maximal in mitosis Mitotic arrest further enhances ROS and oxidative damage to proteins and nucleotides

Techniques: Virus, Recombinant, Software